Anti-LHPP Antibody Picoband™

Product Name

Anti-LHPP Antibody Picoband™

View all LHPP Antibodies

SKU/Catalog Number

A10687

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-LHPP Antibody Picoband™ catalog # A10687. Tested in ELISA, IF, ICC, WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-LHPP Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10687)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Clone Number

1B9

Isotype

IgG

Immunogen

E.coli-derived human LHPP recombinant protein (Position: K6-K270).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A10687 is reactive to LHPP in Human, Mouse, Rat

Applications

A10687 is guaranteed for ELISA, Flow Cytometry, IF, ICC, WB Boster Guarantee

Observed Molecular Weight

29 kDa

Calculated molecular weight

29.165kDa

Background of LHPP

LHPP, also known as HDHD2B, is a 270 amino acid protein that exists as a homodimer and is a member of the HAD-like hydrolase superfamily. Expressed in liver, kidney and moderately in brain, LHPP is encoded by a gene located on 10, which houses over 1,200 genes and comprises nearly 4.5 % of the human genome. Defects in some of the genes that map to chromosome 10 are associated with Charcot-Marie Tooth disease, Jackson-Weiss syndrome, Usher syndrome, nonsyndromatic deafness, Wolman?s syndrome, Cowden syndrome, multiple endocrine neoplasia type 2 and porphyria

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 µg/ml, Human
Flow Cytometry (Fixed), 1-3 µg/1x10⁶ cells, Human
ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of LHPP using anti-LHPP antibody (A10687).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A549 whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human A431 whole cell lysates,
Lane 4: human HepG2 whole cell lysates,
Lane 5: human Hacat whole cell lysates,
Lane 6: human MCF-7 whole cell lysates,
Lane 7: human HEL whole cell lysates,
Lane 8: rat RH35 whole cell lysates,
Lane 9: mouse HEPA1/6 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LHPP antigen affinity purified polyclonal antibody (Catalog # A10687) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LHPP at approximately 29 kDa. The expected band size for LHPP is at 29 kDa.

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Figure 2. IF analysis of LHPP using anti-LHPP antibody (A10687).
LHPP was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-LHPP Antibody (A10687) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

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Figure 3. Flow Cytometry analysis of JK cells using anti-LHPP antibody (A10687).
Overlay histogram showing JK cells stained with A10687 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-LHPP Antibody (A10687, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For LHPP (Source: Uniprot.org, NCBI)

Alternative Names

FLJ44846; HDHD2B; hLHPP; MGC117251; MGC142189; MGC142191; phospholysine phosphohistidine inorganic pyrophosphate phosphatase LHPP HDHD2B phospholysine phosphohistidine inorganic pyrophosphate phosphatase phospholysine phosphohistidine inorganic pyrophosphate phosphatase|hLHPP

For more info on LHPP, check out the LHPP Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for LHPP: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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